Estimating Asian Carp abundance using environmental DNA

Grant: # 1197

Grant Amount: $249,999.00

Board Decision Year: 2011

University of Notre Dame - Center for Aquatic Conservation & Environmental Change Initiative (South Bend)

Center for Aquatic Conservation & Environmental Change Initiative

Lodge, David (dlodge@nd.edu) 574-631-2849

GLFT - Ecosystem Health and Sustainable Fish Populations-D - Ecological and biological fisheries research to inform management

Project Details

This study provides a number of critical insights and advancements. First, because of the direct comparison of qPCR and PCR assays, it is now possible to deploy a more sensitive detection approach (the qPCR assay) to the surveillance of bighead and silver carp. Second, it is clear that the PCR assay used from 2009 to present in the CAWS and Great Lakes is more prone to false negatives than false positives.  Third, while there is a positive relationship between Asian carp abundance and amount of DNA in the water, there is sufficient variability to make estimation of abundance from amount of DNA very difficult. Fourth, environmental conditions drive fluctuations in the amount of DNA present. Overall, considering previous PCR detections, the implications of false negatives, the screening of the CAWS using a new qPCR, Asian carp marker, and comparing eDNA concentrations we measured in the CAWS relative to those we measured in our experimental ponds, the most reasonable inference is that some Asian carp are present on the Lake Michigan side of the electric barrier in the CAWS.

Documents

Final Report Narrative
View - Lodge_et_al_eDNA_Final_Report_to_GLFT.pdf
2011.1197 Project Summary
View - 2011.1197_Lodge_Project_Summary.pdf